You're right Jeanette, This has been discussed repeatedly, and sometimes it generates a lot of heat. But we can handle that. There is some good material on the various CDs released by Paul Robinson and his associates, should you have access to them. I was impressed by Philippe Poncelet's analysis of these issues last september. He can be contacted as indicated: Philippe Poncelet, PhD Scientific Director, BioCytex Marseilles - France Tel:+33 - 4 91 94 29 39 Fax:+33 - 4 91 47 24 71 E-mail: Still my boss's one: mcanton@wcube.fr I can also copy to you, some of that correspondence, the balance of which Philippe may have. The 2 papers I quote are the EMBO J, 1986: Chatelier et al; Vol5 1181-1186 and the Cytometry, 1987 Chatelier and Ashcroft Vol 8, 632-636. Let me know if you need more. Arrividerci Gianetta! -----Original Message----- From: Jeanette Drew [SMTP:jdrew@cyllene.uwa.edu.au] Sent: Wednesday, November 19, 1997 10:22 PM To: Cytometry Mailing List Subject: Quantitative measurement of cell surface molecules Hello fellow flowers, I am deferring a request received for info (see below) that after a quick scan of my references and methods texts reveals that I do not have any literature on the quantitative measurement of cell markers by FCM, although I know literature does exist, albeit from memory controversial. This was discussed on this list a few months ago with Bob Ashcroft a primary respondent, so I would appreciate your comments Bob, and any others to forward back to my colleage. Many thanks, Jeanette. Do you know how you can calculate the number of molecules on the surface of a cell using flow? I'm sure it's been done by lots of people so if it's easy we would like to do it. As you're the only person I know that works with these machines I thought you might know where to look! Ciao Ciao Bernadette xxxx _______________________________________________________________ Jeanette Drew Department of Obstetrics & Gynaecology University of Western Australia / King Edward Memorial Hospital SUBIACO WA 6009 AUSTRALIA Tel: 61-8-9340-2768 ,-_|\ Pager: 61-8-9485-6570 / \ Fax: 61-8-9340-2636 JD -> *_,-._/ Email: jdrew@cyllene.uwa.edu.au v _______________________________________________________________
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