Dear Gregg, What antigens do you assay for? What quantity of antigen is on the beads (as/mg of beads)? Are your results quantitative, or just +ve or -ve? What is the assay detection limit for each antigen? Just a small sample of qq that would be useful. Thanks, Bob -----Original Message----- From: ridder.gm@pg.com [SMTP:ridder.gm@pg.com] Sent: Saturday, November 15, 1997 1:22 AM To: Cytometry Mailing List Subject: Re[2]: Luminex technology We are working with Luminex and have a very different view of them. The Coulter machines simply represent a technical interface problem for on-line analysis; thus they are reluctance to utilize this machine temporarily until their own machine comes out. However, we use a Coulter Elite ESP to gather the listmode files and Luminex is modifying their software for batch off-line analysis; they have been very accommodating and we see no technical limitations. We visited them in Austin for two days to develop an assay and run our own samples on their equipment. Running standards for 9 simultaneous analyses worked as advertised; it was fast and simple. There should be significant cost savings over ELISA assays, but the exact prices of FlowMetrix kits under development are not established yet. The sensitivities were not quite as good as our standard ELISA's, but from other literature on bead analyses and their previous experience with other assays I believe it could be. We had difficulty with high backgrounds in running our samples as a washless assay and both our labs are in the process of tweaking the method. Their new cytometer is very innovative and may set some new standards in cost and design. In all, we remain excited by the possibilities, see no fundamental limitations and will pursue this avenue. But, we're not there yet. (We have no commercial interest in Luminex Corp) Gregg M. Ridder Miami Valley Labs Procter & Gamble PO Box 538707 Cincinnati, OH 45253-8707 ridder.gm@pg.com Phone: (513) 627-2634 FAX: (513) 627-1760 >No, But I can tell you there was hesitancy from the personnel at Luminex >about doing studies on a Coulter flow cytometer when I offered such at the >CAC meeting in August. The technology looks exciting, but Luminex remains >circumspect. > ---------- >From: Gregor Rothe >To: cyto-inbox >Subject: Luminex technology >Date: Thursday, November 06, 1997 5:44PM The Austin (TX) based company LUMINEX claims that their microbead technology allows the simultaneous analysis of 64 parameters in flow cytometric multi-ligand assays in immunology or molecular biology. Is their anyone on this list who can comment based on practical experiences with different applications using their products? Thanks for any information, Gregor Rothe *********************************************************** Institute for Clinical Chemistry and Laboratory Medicine University of Regensburg D-93042 Regensburg, Germany Tel. +49 (941) 944-6204 Fax +49 (941) 944-6202 E-mail: Gregor.Rothe@KLINIK.UNI-REGENSBURG.DE ***********************************************************
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