CCR5 Staining

From: Steve Perfetto (sperfetto@hiv.hjf.org)
Date: Thu Nov 13 1997 - 17:12:39 EST

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To all,

RE: CCR5 MAB staining;

I would like to know if anyone has experience staining HOS (transfected with
CCR5 gene) cells or stimulated PBMC's (PHA/IL2) using R&D systems CCR5 clones. 
We have tried these clones with various fixing conditions and have not been
successful.  If anyone has a protocol for these clones I would like to try it.

Thanks

Stephen P. Perfetto, MS.,MT. (ASCP)
Walter Reed Army Institute of Research
Department of Diagnostic Retrovirology
1600 East Gude Drive
Rockville, MD. 20850

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