We have similar problem since initially we have an old Coulter, then I used
Verify that allow to change the files from coulter list mode into FCS
files. From FCS files I was able to do everything that I need but only by
using Lysis o PC-lysis. I try several times with Winmdi but the best that I
could get is flat histograms and neither dot nor contours, and of course
all the statistics is lost. I am not fond of BD sofware (very expensive)
and because winmdi is free, it is the easy way to go. However, I am not a
computerfreak (still I read word and email for dummies) so I could not get
all the advantges from winmdi. May be Mario Roederer from Stanford
(Roederer@Beadle.Stanford.EDU) could help you better with his new software.
Regards and good luck
Rafael
>I am sending this message on behalf of Rohan Ameratunga. Please send
>replies directly to him at:
>
>
>Many Thanks
>Jan
>
>Dear flow cytometry colleagues,
>
>I would appreciate advice on a technical problem I have recently
>struck. I have recently downloaded a copy of Winmdi 2.5 and I am
>attempting to use listmode files saved on a Coulter Epics flow
>cytometer. Unfortunately the program wont open the files.
>
>Is anyone aware of a conversion utility to deal with this problem? I
>would appreciate any help on this matter.
>
>Best regards
>
>Rohan Ameratunga
>Immunologist
>
>Jan Nelson
>Molecular Haematology
>Department of Molecular Medicine
>The University of Auckland
>Phone: (+64)(9) 373-7599 ext 6381
>Fax: (+64)(9) 373-7292
\|/
(o o)
________________________________oOo__(_)__oOo_________________________________
___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch
/ o \/| | University Inst.for Virology http://www.unizh.ch/vetvir
/ _| | Winterthurerstr. 266a Telephone: (+41) 1 6358710
/_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911
______________________________________________________________________________
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