Hello everyone. I have a question pertaining to a phenomenon I have observed with my Coherent 621 laser (produces 488 & UV simultaneously). I'm using a BD FACSVAntage with TSO and am looking at the 621's intercept using the video monitor. The laser is using house water for cooling and the temperature of the water is about 60 degrees F. I have noticed that when I fire the laser up in the morning the intercept is at 47 (arbitrary video unit) and after about 3 hours of warming up the intercept has moved down the stream to 53. Has anyone else seen this intercept movement in these lasers? Doesn't this movement mean problems for proper drop timing? Is 3 hours a normal warmup time for a laser such as this? Thanks - may the sort be with you, Barry Barry Grimes FACS Lab Manager Hematopoiesis Center Markey Cancer Center 800 Rose St. cc418 Lexington,KY 40536-0093 lab) 606-323-8193 fax) 606-257-7715 e-mail) bagrim1@pop.uky.edu
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