Flowers, Just a note to follow up on the outcome of my problem with an apparent "laser drift" (see original post below) in my Enterprise 621 laser attached to a BD FACSVantage. The problem was ultimately diagnosed by our excellent BD Service Engineer as a video/monitor problem. The replacement of the "PCB ASSY, Bench Control" board has rectified any movement of the monitor viewing markers. Thanks to all who responded - especially Cornelia in Dresden Germany. >> Hello everyone. I have a question pertaining to a phenomenon I have >> observed with my Coherent 621 laser (produces 488 & UV simultaneously). I'm >> using a BD FACSVAntage with TSO and am looking at the 621's intercept using >> the video monitor. The laser is using house water for cooling and the >> temperature of the water is about 60 degrees F. >> >> I have noticed that when I fire the laser up in the morning the intercept >> is at 47 (arbitrary video unit) and after about 3 hours of warming up the >> intercept has moved down the stream to 53. >> >> Has anyone else seen this intercept movement in these lasers? Doesn't this >> movement mean problems for proper drop timing? Is 3 hours a normal warmup >> time for a laser such as this? Barry Grimes FACS Lab Manager Hematopoiesis Center Markey Cancer Center 800 Rose St. cc418 Lexington,KY 40536-0093 lab) 606-323-8193 fax) 606-257-7715 e-mail) bagrim1@pop.uky.edu
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