Hi Dennis At 09:39 AM 6/11/97, you wrote: >We find that phenol red is not a problem. We sort cells _stained_ (FITC + >PE +Cy5-PE) in media with phenol red. I'll bet the vitamins in the media >(that then accumulates in the cells) contribute more to autofluorescence >than the phenol red! You're probably correct; my observations are that cultured cells often give me this kind of problem, and the common thing has been culture media containing some fluorescent indicator. I assumed the obvious, and have not bothered to find out what else is in the media. Thanks for adding to my education, I'll extract the hoof from the intake and see if we can investigate further.. Cheers, Joseph. -- Joseph Webster Flow Cytometry Facility Centenary Institute
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:50:19 EST