Dear all, We are testing some monoclonals developed in an affiliated lab against myelogenous leukemia. We are trying to see if they are specific against a particular sub-classification of AML (FAB M0 to M7), labeling the cells with the monoclonal followed by the appropriate conjugate plus a set of commercial monoclonals (CD7, CD13, CD14, CD19 and CD34). Our problem is the high background fluorescence the cells are presenting on incubation with the isotype controls, which are making the interpretation of the results quite difficult. Is there any other suggestion besides pre-incubating the cells with media plus FCS?. Any tips are welcome. Thanks in advance. Jorge Neumann Lab Transplantation Immunology Santa Casa Hospital Porto Alegre, Brasil
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