Flowers
Thanks to all the people who replied to my question. Thanks especially
(as usual) to Howard Shapiro who supplied a list of references that kept
our library busy for several days, where does he find the time to do
everything. As usual the method is very easy if one knows how and I
should have been able to work it out for myself. There are basically
two ways to do this, add a label for RBCs and gate them out, or add a
pan WBC marker and trigger on fluorescence, so that the instrument never
knows the red cells are there. Of course they are there and the files
will be large and the collection time will be long, on a bench top
cytometer, but as we always say in core facilities " my time is your
money" Thanks again to you all,
Jill
Jill Martin, , Manager
Molecular Biology & Flow Cytometry Core
Johnson Research Building
Mayo Clinic Scottsdale
602-301-6071 (Voice)
602 301-7017 (FAX)
email: jmartin@mayo.edu or martin.jill@mayo.edu
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