Re: Staining of Whole Blood.

From: Simon Monard (Simon_Monard@adarc.org)
Date: Thu Oct 16 1997 - 14:24:39 EST

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Hi Jill how's things

Staining whole blood is easy, add 100u blood to each tube, add your
antibodies, incubate, then add 1ml of a lysing reagent, I use BDs hugely
expensive FACSlyse, wait 10 minutes then wash a couple of times and run. I
use it all the time for 4 color staining, works fine and is very quick. Is
this what you meant?

Simon
Freezing cold New York

Next message: Brendel Cornelia: "Sorting of human WBC after freezing"
Previous message: erik a gamerl: "B cells"
Maybe in reply to: Martin, Jill V.: "Staining of Whole Blood."
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