Dear Dr. Cabrera, I haven't flowed pig WBC, but cow, cat, sheep, and hamster. If you don't want to Ficoll your cells (dilute your blood 1:2 or 1:3), you can try to use an NH4Cl-based lysing solution. An easy to prepare is: 8.99 g/L NH4Cl, 1g/L KHCO3, 37 mg/L tetrasodium EDTA, pH 7,3. Add 7 ml of lysing solution to 500 microliter of your blood, mix gently and when RBC will are lysed spin for 7 minutes. Wash 2x in saline or PBS and resuspend in your staining media. I usually stain 10 samples starting from 500 microliters of blood (cow PMN phenotyping). Hope this help Mara ---------- Mara Rocchi, DVM, Ph.D. International Livestock Research Institute Nairobi, Kenya Fax: 00254-2-631499 e-mail: M.Rocchi@cgnet.com
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