Dear Group, I have occasionally recovered archival PB CD34 cells from the freezer to use in some CD34 selection/expansion experiments. The usual cell clumping problems are always present and contribute to very high cell losses ( ... cest la vive). I have been thinking about including some DNAse into my thawing buffer to try and minimise this problem (I am assuming that free DNA from disintegrated cells is the major culprit in the whole process). Has anyone in the group tried this ??? what final concentration of DNAse should I aim at ?? Thanks in advance Peter Chapple Melbourne AUSTRALIA
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