I have recently compared PI staining with ToPro3 on bugs and
saw exactly the same. Whilst at low concentration only dead
cells appear highly fluorescent, at high concentrations all
bacteria lit up with the dead ones about 10 times higher.
Admittingly I haven't looked down the microscope yet to see
where the dye goes, so I would also be quite interested in
the explanation.
Gerhard.nebe-von-caron@unilever.com
______________________________ Reply Separator _________________________________
Subject: SYBR-14 binding mechanism?
Author: mbochen@izoo.krakow.pl at INTERNET
Date: 30/09/97 21:45
Hi, everybody
Live cells stained with SYBR-14 (DNA stain, penetrates intact
membranes) fluoresces brighter (ab. 10x) than dead ones. Why does it happen?
Or, in the other words: what is the mechanism by which SYBR-14 binds to DNA?
Saying 'cells' I mean spermatozoa. I don't know what about other kinds
of cells.
Thanks in advance for any information.
Michal
=============================================================================
| National Research Institute of Animal Production
Michal Bochenek | Department of Animal Reproduction
mbochen@izoo.krakow.pl | 32-083 Balice / Krakow, Poland
| phone: 48 12 2856777 ext.145; fax: 48 12 2856733
| e-mail: zfr@izoo.krakow.pl
|
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