SYBR-14 binding mechanism?

From: Michal Bochenek (mbochen@izoo.krakow.pl)
Date: Tue Sep 30 1997 - 04:00:14 EST

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Hi, everybody

Live cells stained with SYBR-14 (DNA stain, penetrates intact 
membranes) fluoresces brighter (ab. 10x) than dead ones. Why does it happen?
Or, in the other words: what is the mechanism by which SYBR-14 binds to DNA?

Saying 'cells' I mean spermatozoa. I don't know what about other kinds 
of cells.

Thanks in advance for any information.
Michal

=============================================================================
                       |  National Research Institute of Animal Production
Michal Bochenek        |         Department of Animal Reproduction
mbochen@izoo.krakow.pl |          32-083 Balice / Krakow, Poland
                       |   phone: 48 12 2856777 ext.145; fax: 48 12 2856733
                       |            e-mail: zfr@izoo.krakow.pl
                       |
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