kcdol@samsung.co.kr wrote: > > Dear collegues > > I wonder how many laboratories do the nuclear TdT stain > by flow cytometric method after membrane permeabilization > not by cytospin followed by fluorscence microscopic reading? > > In case of flow cytometric method, which protocol for membrane > permeabilization you use? > Is the permeabilization reagent Triton-X, saponin, FACS Lysing Solution, > FACS Permeabilization Solution, Fix&Perm, or something special? > > And, have you ever tried to compare the results between > flow cytometric method and cytospin method? > > Any comments will be appreciated. > Thanks in advance. > > --------------------------------- > Chang-Seok Ki, M.D. > Dept. of Clinical Pathology > Samsung Medical Center > Seoul, Korea > Tel. 82-2-3410-2708 > Fax. 82-2-3410-2719 > E-mail. kcdol@samsung.co.kr > --------------------------------- We use to investigate nuclear Tdt expression by flow cytometry with a permeabilization protocol. We have used orthopermeafix and Perm and Fix and they compare well. A collegue of mine is currently developing a permeabilization protocol associating tryton and paraphormaldeyde with excellent results in terms of detection. Adriano Venditti Cattedra di Ematologia-Università di Roma "Tor Vergata" tel.+39-6-59044101 fax.+39-6-5915965 E-mail.avenditti@pelagus.it
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