Dear collegues I wonder how many laboratories do the nuclear TdT stain by flow cytometric method after membrane permeabilization not by cytospin followed by fluorscence microscopic reading? In case of flow cytometric method, which protocol for membrane permeabilization you use? Is the permeabilization reagent Triton-X, saponin, FACS Lysing Solution, FACS Permeabilization Solution, Fix&Perm, or something special? And, have you ever tried to compare the results between flow cytometric method and cytospin method? Any comments will be appreciated. Thanks in advance. --------------------------------- Chang-Seok Ki, M.D. Dept. of Clinical Pathology Samsung Medical Center Seoul, Korea Tel. 82-2-3410-2708 Fax. 82-2-3410-2719 E-mail. kcdol@samsung.co.kr ---------------------------------
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