As I have indicated (and published) before, Orthopermeafix does not work for TdT detection in AML; Fix & Perm works well in ALL and AML. E. Paietta On Fri, 12 Sep 1997, adriano venditti wrote: > > kcdol@samsung.co.kr wrote: > > > > Dear collegues > > > > I wonder how many laboratories do the nuclear TdT stain > > by flow cytometric method after membrane permeabilization > > not by cytospin followed by fluorscence microscopic reading? > > > > In case of flow cytometric method, which protocol for membrane > > permeabilization you use? > > Is the permeabilization reagent Triton-X, saponin, FACS Lysing Solution, > > FACS Permeabilization Solution, Fix&Perm, or something special? > > > > And, have you ever tried to compare the results between > > flow cytometric method and cytospin method? > > > > Any comments will be appreciated. > > Thanks in advance. > > > > --------------------------------- > > Chang-Seok Ki, M.D. > > Dept. of Clinical Pathology > > Samsung Medical Center > > Seoul, Korea > > Tel. 82-2-3410-2708 > > Fax. 82-2-3410-2719 > > E-mail. kcdol@samsung.co.kr > > --------------------------------- > We use to investigate nuclear Tdt expression by flow cytometry with a > permeabilization protocol. We have used orthopermeafix and Perm and Fix > and they compare well. A collegue of mine is currently developing a > permeabilization protocol associating tryton and paraphormaldeyde with > excellent results in terms of detection. > Adriano Venditti > Cattedra di Ematologia-Università di Roma "Tor Vergata" > tel.+39-6-59044101 > fax.+39-6-5915965 > E-mail.avenditti@pelagus.it >
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