In my experience DMSO preservatives do indeed lead to Annexin-V positivity. This seems to be the case with both tumour FNA samples and trypsinised cell lines. The preservative I use is the standard freezing mixture, 10% DMSO in fetal calf serum. Eric P Miller Edinburgh Medical Oncology Unit "Wouldn't it be nice if hospitals and schools had all the money they needed and the army had to hold jumble sales to buy guns?" On Thu, 11 Sep 1997, Peter Kierulf wrote: > > Please post the following message: > > "Anyone know anything about DMSO-based cryopreservation , does this > lead to cells becoming Annexin V positive? > > Thanks. By the way your service is excellent > > Peter Kierulf,MD PhD. Professor I > Clinical Chemistry Dep. > Ulleval University Hospital > N-0407 OSLO, NORWAY > > Telephone: +47 22 11 94 86 > Telefax: +47 22 11 81 89 > _-----------------------------------Mobil telphone: 922.85111 > E-mail (RFC Formate): peter.kierulf@ioks.uio.no > E-mai. (SA Formate): /G=peter/S=kierulf/OU=ioks/O=uio/PRMD=uninett/C=no > > > Peter Kierulf,MD PhD. Professor I > Clinical Chemistry Dep. > Ulleval University Hospital > N-0407 OSLO, NORWAY > > Telephone: +47 22 11 94 86 > Telefax: +47 22 11 81 89 > _-----------------------------------Mobil telphone: 922.85111 > E-mail (RFC Formate): peter.kierulf@ioks.uio.no > E-mai. (SA Formate): /G=peter/S=kierulf/OU=ioks/O=uio/PRMD=uninett/C=no > > >
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