> In my experience DMSO preservatives do indeed lead to Annexin-V > positivity. This seems to be the case with both tumour FNA samples and > trypsinised cell lines. The preservative I use is the standard freezing > mixture, 10% DMSO in fetal calf serum. > > Eric P Miller > Edinburgh Medical Oncology Unit > > "Wouldn't it be nice if hospitals and schools had all the money they > needed and the army had to hold jumble sales to buy guns?" > Also in my experience with cryopreserrved tissue i have observed about 30-40% of cells positiv to Annexin-V.What is your opinion about the mechanism bay witch DMSO inicjate the inverse of cell membrane. Is this really a apoptotic process. Could your recomend me some materaials (publications) about this. Piotr Wilczek, MSc Foundation of Cardiac Surgery Development tel.: (+48 32) 271-06-31 ext. 118, 131 fax: (+48 32) 271-38-00 piotr@serce.fcsd.gliwice.pl
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