An investigator in our Institute asked me a question that I'd like to pass along: He is doing a multicolor sorting experiment and wants to remove the fluorochrome labeled antibody after sorting, before he puts the cells into culture. The only thing I could think of was trypsin treatment, but I was unsure of the long term effects of this, or whether this would cause more harm than good. Any suggestions? Thanks. Kevin L. Holmes, Ph.D. Head, Flow cytometry Unit Office of the Scientific Director Bldg 7, Room 01 NIAID, NIH Phone: 301-496-9071 FAX: 301-402-4532 Email: kholmes@atlas.niaid.nih.gov
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