At 01:02 PM 8/13/97 +0000, you wrote: > >Cytometrists, >I have two interesting cases that I would like some help with. >1. The first patient appears to be a new acute leukemia with very >large irregular cells. The bone marrow phenotypes CD13+,CD15+,CD33+,HLA DR+, >CD45+(bright),CD14 My4+ but CD14 Mo2 negative. >I would like to know others' experience with My4. Does it mark a >more immature monocytoid cell? Would you call this myelomono- >leukemia? dear michaeleen i dont know the difference in epitope specificity between the two CD14 clones, but if one is positive,and there is a positive (cytochemical) esterase reaction there is certainly evidence for an "M5" variety monocytic leukemia. Is there 'dim' CD4? that is another tip to monocytic differentiation >2. The second case is a patient with normal numbers of all cell >types in the peripheral blood but with two clearly different >populations of CD5+ cells--one very bright and the other dimmer but >still positive. They do not dual stain with B cell markers. In >1993 the patient showed a T cell beta gene rearrangement. If we had >not been aware of this we may not have worried so much about the >staining. Has anyone noticed this pattern of CD5 reactivity? I am very familiar with this pattern. The dim CD5+ are most likely CD3+CD8+, the bright are mostly CD3+CD4+. regards ```````````````````````````````````````````` Dr. Frederic I. Preffer preffer@helix.mgh.harvard.edu Department of Pathology- Warren 525A 100 Blossom St Massachusetts General Hospital Boston MA 02114 v(617) 726-7481 fax(617) 724-3164
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