('binary' encoding is not supported, stored as-is) Dear Michaeleen, 1. We have used two different CD14 Mabs on many AML4s with monocyte differentiation : CD14 clone TUK4 and CD14 cloneMEM18. We find it very seldom thet both of them are positive. The imunophenotype you give can very well be consistent with a monocytic leukemis (M5a or b according to FAB). 2.CD5 is quite often "dim" in lymphoproliferative T cell disorders . Could you characterize these cells further ? were they all CD4 or CD8 positive? what about other T-cell markers as CD2,7 and CD3 membr. and cytoplasmic? What about TCR gamma and delta? Withe best wishes Anna Porwit-MacDonald, MD, PhD Heamatopathology Lab. Karolinska Hospital Stockholm >Cytometrists, >I have two interesting cases that I would like some help with. >1. The first patient appears to be a new acute leukemia with very >large irregular cells. The bone marrow phenotypes CD13+,CD15+,CD33+,HLA DR+, >CD45+(bright),CD14 My4+ but CD14 Mo2 negative. >I would like to know others' experience with My4. Does it mark a >more immature monocytoid cell? Would you call this myelomono- >leukemia? >2. The second case is a patient with normal numbers of all cell >types in the peripheral blood but with two clearly different >populations of CD5+ cells--one very bright and the other dimmer but >still positive. They do not dual stain with B cell markers. In >1993 the patient showed a T cell beta gene rearrangement. If we had >not been aware of this we may not have worried so much about the >staining. Has anyone noticed this pattern of CD5 reactivity? >Thank you very much for your help. > >Michaeleen M. Collins > >
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