What I have routinely done is with 2 forceps, grab the main part of the femur with one, and grasp the patella area with the other. Snap apart gently. This wil leave a much more open area from which you need only aspirate media. P. Echeagaray Janet L. Jacobitz wrote: > > On Mon, 11 Aug 1997 17:20:23 -0700 (PDT) you wrote: > > In order to release most of the marrow cells from possible bone marrow > particles we have for years used repeated aspiration of the aspirate through > a small gauge needle (25 ga.). This seems to work fine, does not appear > to damage the cells much and is fairly rapid. It is however not a very > safe practice. I would like to stop having the people in the lab have to > use these needles while manipulating the patient bone marrow in the lab. > Does anyone have any better procedures for releasing cells in the > particles? > We use the frosted ends of microscope slides for tissues and this works > fine but is impractical for aspirates. > > Tom McHugh > Dept. Lab. Medicine > UCSF > mchugh@labmed.ucsf.edu > > We routinely use a sterilized fine mesh screen placed over a petri dish, > pull the plunger out of a 12 ml disposable syringe, and use it to push the > marrow through the screen. Use media to flush the screen. The screen can be > purchased from Small Parts Inc and is called wire cloth type 304 ( or you > may use a smaller mesh if desired). > ********************************** > * Jan Jacobitz * > * C-V Surgery Lab Manager * > * Children's Memorial Hospital * > * 2300 Children's Plaza Box 220 * > * Chicago,IL 60614 * > * phone: 773-880-4676 * > * fax: 773-880-3088 * > * e-mail:jjacobitz@nwu.edu * > **********************************
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