On Mon, 11 Aug 1997 17:20:23 -0700 (PDT) you wrote: In order to release most of the marrow cells from possible bone marrow particles we have for years used repeated aspiration of the aspirate through a small gauge needle (25 ga.). This seems to work fine, does not appear to damage the cells much and is fairly rapid. It is however not a very safe practice. I would like to stop having the people in the lab have to use these needles while manipulating the patient bone marrow in the lab. Does anyone have any better procedures for releasing cells in the particles? We use the frosted ends of microscope slides for tissues and this works fine but is impractical for aspirates. Tom McHugh Dept. Lab. Medicine UCSF mchugh@labmed.ucsf.edu We routinely use a sterilized fine mesh screen placed over a petri dish, pull the plunger out of a 12 ml disposable syringe, and use it to push the marrow through the screen. Use media to flush the screen. The screen can be purchased from Small Parts Inc and is called wire cloth type 304 ( or you may use a smaller mesh if desired). ********************************** * Jan Jacobitz * * C-V Surgery Lab Manager * * Children's Memorial Hospital * * 2300 Children's Plaza Box 220 * * Chicago,IL 60614 * * phone: 773-880-4676 * * fax: 773-880-3088 * * e-mail:jjacobitz@nwu.edu * **********************************
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