There is a letter to the editor in BLOOD , September 1996, 15:88(6):23558-61 by van Vugt, MJ et al which shows that PE-CY5 binds SPECIFICALLY to the Fc(gamma)R1 receptor (CD64). These experiments using mouse B cells transfected with different human Fc receptors showed that only FcR1 cells bound PE-CY5. Whole blood showed much less binding than isolated cells. Their recommendation was that any antibody that you are using labelled with PE-CY5 be tested for FcR1 binding. In our hands blocking with heat inactivated normal human AB serum will significantly reduce the Fc binding in humans. Mike Keeney London Health Sciences Centre London Ontario Canada
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