Hi, I am wondering if I should expect any problems with GFP fluorescence if I stain my cells in a solution of PI (50ug/ml), Triton X-100 (0.1%), and sodium citrate (0.1%) overnight at 4'C. I would also appreciate any general information on the compatibility (or incompatibility, as I understand it) of GFP with fixatives (formaldehyde, ethanol, etc.). Thanks, Michele Houston Montefiore Medical Center Department of Oncology
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