Julie, You do not say what you want to stain with this , but
for most mouse tissue my question would be why do you want to use CD45
with APC? The usual method for selecting a fluorochrome is to put the
lowest epitope density together with the brightest Fluorochrome and vice
versa, For most mouse work that means CD45-FITC which leaves the other
three or more colours for the brighter dyes.
( You see Carlton, I do pay attention in class.)
Jill Martin, Manager,
Molecular Biology & Flow Cytometry Core
Johnson Research Building
Mayo Clinic Scottsdale
602-301-6071
email: jmartin@mayo.edu or martin.jill@mayo.edu
>Does anyone know of a supplier of anti-mouse CD45-APC (conjugated to
>another fluorochrome suitable for four colour staining)? We've found a
>biotinylated product, but would prefer a directly conjugated product if
>possible.
>
>Thanks.
>
>Julie
>
>______________________________________________
>Julie Stevenson
>
>Bone Marrow Transplant Lab,
>Children's Cancer Institute of Australia,
>Sydney Children's Hospital
>Randwick, NSW, 2031
>
>Ph 61 2 9382 1819, Fax 61 2 9382 1850
>email julie.stevenson@unsw.edu.au
>______________________________________________
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