Yes, I have seen TdT expression in APL, usually not such a high percentage, though. What anti-TdT antibody was used - a directly conjugated monoclonal? EPaietta On Sun, 3 Aug 1997 kcdol@samsung.co.kr wrote: > > > Dear flowers > > Have you ever experienced TdT positive AML M3? > > The patient was in his 30s and the bone marrow > was clouded with typical M3 promyelocytes. > They expressed surface antigen of CD13+and CD33+ without > HLA-DR. > All lymphoid lineage markers were negative. > Our protocol was as follows; > 1. Mononuclar cell separation with Ficoll-Hypaque > 2. Wash with 1%BSA-PBS once > 3. Permeabilization with FACS Brand Lysing Solution > 4. Wash with 1%BSA-PBS once > 5. Incubation with anti-TdT MAb > 6. Wash with 1%BSA-PBS once > 7. Data aqusition and analysis with FACSort and HP340 > computer system > > Autofluorescence without anti-TdT MAb was used as negative > control and MOLT-3 cell line was used as positive control. > > About over 90 percent of promyelocytes were nuclear TdT > positive. > > What's wrong or was it real? > > Chang-Seok Ki, MD > Dept. of Clinical Pathology > Samsung Medical Center > >
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