Hi, I would appreciate any help that I can get on doxorubicin retention assays. I am performing rhodamine efflux assays routinely but I can't get the doxo retention assay going. I get excellent uptake but then the doxo stays within the cells even over a 24 hour period. I have tried various incubation temperatures and I am using P-glycoprotein positive cells by rhodamine efflux assay. Any ideas? Thanks, E. Paietta
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