Dear flowers Have you ever experienced TdT positive AML M3? The patient was in his 30s and the bone marrow was clouded with typical M3 promyelocytes. They expressed surface antigen of CD13+and CD33+ without HLA-DR. All lymphoid lineage markers were negative. Our protocol was as follows; 1. Mononuclar cell separation with Ficoll-Hypaque 2. Wash with 1%BSA-PBS once 3. Permeabilization with FACS Brand Lysing Solution 4. Wash with 1%BSA-PBS once 5. Incubation with anti-TdT MAb 6. Wash with 1%BSA-PBS once 7. Data aqusition and analysis with FACSort and HP340 computer system Autofluorescence without anti-TdT MAb was used as negative control and MOLT-3 cell line was used as positive control. About over 90 percent of promyelocytes were nuclear TdT positive. What's wrong or was it real? Chang-Seok Ki, MD Dept. of Clinical Pathology Samsung Medical Center
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:58 EST