Hi folks, Many thanks to those who responded re my laser troubles: we had a bad 15VDC power supply, and once I swapped that we were back in business. Now I am faced with researchers inquiring about the feasibility of two different projects, and I can't find much in the literature. We're still looking, but any first-hand experiences or tips would be greatly appreciated--I'm not sure either of these are feasible: a) Subject A wants to hybridize oligos to specific RNA sequences, to detect levels of expression of specific genes. I'm aware of methods for measuring mRNA content by flow, but not of specific genes. I have feeling our signal would be verrry dim. b) Subject B has already collected (! arrgghh !) spleen and bone marrow samples from mice exposed to high levels of radiation, and would like to do micronuclei assays or chromatin fragmentation. The Nusse et al chapter in the "D.R.C., Methods in Cell Bio. vol II" seems promising, but only with cell cultures. Primary tissue apparently has a lot of debris, which confuses the detection of micronuclei. I would also think that this will be especially difficult with heterogeneous tissue like bone marrow. Anyone have any encouraging words....anyone? Thanks in advance, Steve PS--I saw the message from Peter, but has anyone heard from the folks at CSU? Best wishes and my sympathy to everyone in Fort Collins! ------------------------------------------------------------- Steve G. Hilliard Linux: In a world Cell Analysis Facility without fences, University of Georgia who needs Gates? -------------------------------------------------------------
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