Two hairy proposals

From: Steve G. Hilliard (steve@habanero.cb.uga.edu)
Date: Wed Jul 30 1997 - 11:15:31 EST


Hi folks,

Many thanks to those who responded re my laser troubles:  we had a bad 
15VDC power supply, and once I swapped that we were back in business.  
Now I am faced with researchers inquiring about the feasibility 
of two different projects, and I can't find much in the literature.  
We're still looking, but any first-hand experiences or tips would be 
greatly appreciated--I'm not sure either of these are feasible:

a)  Subject A wants to hybridize oligos to specific RNA sequences, to 
detect levels of expression of specific genes.  I'm aware of methods for 
measuring mRNA content by flow, but not of specific genes.  I have 
feeling our signal would be verrry dim.

b)  Subject B has already collected (! arrgghh !) spleen and bone 
marrow samples from mice exposed to high levels of radiation, and 
would like to do micronuclei assays or chromatin fragmentation.  The 
Nusse et al chapter in the "D.R.C.,  Methods in Cell Bio. vol II" seems 
promising, but only with cell cultures.  Primary tissue apparently has 
a lot of debris, which confuses the detection of micronuclei.  I would 
also think that this will be especially difficult with heterogeneous 
tissue like bone marrow.

Anyone have any encouraging words....anyone?

Thanks in advance,
Steve

PS--I saw the message from Peter, but has anyone heard from the folks at 
CSU?   Best wishes and my sympathy to everyone in Fort Collins!

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Steve G. Hilliard                   Linux:   In a world 
Cell Analysis Facility                 without fences, 
University of Georgia                  who needs Gates?
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