To followup to my own post, I apologize about subject B--we have since discovered quite a few papers dealing with in vivo tissues. That's what I get for relying on someone else's lit search! Any suggestions still gratefully accepted! On Wed, 30 Jul 1997, Steve G. Hilliard wrote: > > b) Subject B has already collected (! arrgghh !) spleen and bone > marrow samples from mice exposed to high levels of radiation, and > would like to do micronuclei assays or chromatin fragmentation. The > Nusse et al chapter in the "D.R.C., Methods in Cell Bio. vol II" seems > promising, but only with cell cultures. Primary tissue apparently has > a lot of debris, which confuses the detection of micronuclei. I would > also think that this will be especially difficult with heterogeneous > tissue like bone marrow. > ------------------------------------------------------------- Steve G. Hilliard Linux: In a world Cell Analysis Facility without fences, University of Georgia who needs Gates? -------------------------------------------------------------
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