> Has anyone tried this combination? Or are there any other > apoptosis markers that would work with GFP? We haven't specifically tried GFP with annexin, but there is no reason for this not to work--we often use GFP with a wide variety of fluorochromes, like PE, Cy5PE, TR, APC, etc; annexin V can be conjugated to any of these to be used in combination with GFP. If you have the annexin V protein (it can be obtained both commercially and in collaboration with some scientists producing recombinant versions), conjugation instructions can be found at "http://cmgm.stanford.edu/~roederer/abcon/". BTW, the annexin V staining survives 0.5% paraformaldehyde fixation just fine. mr
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:57 EST