GFP/Apoptosis

From: ronald hill (ronald@sugen.sf.ca.us)
Date: Mon Jul 28 1997 - 15:09:45 EST


Greetings,
We are interested in monitoring apoptosis in cells that are 
co-transfected with Green Fluorescent Protein (GFP) as a marker of 
transfection. We currently use PI staining to measure apoptosis, but 
evidently the fixation method is not compatible with GFP methods. We 
have thought of using Annexin V to monitor apoptosis instead. Has 
anyone tried this combination? Or are there any other apoptosis 
markers that would work with GFP?
Thanks in advance.
-- 
Ronald J. Hill, Ph.D.
Research Scientist
Sugen, Inc.
515 Galveston Dr.
Redwood City, CA 94063

ronald@sugen.sf.ca.us
tel #: 415-306-7797
fax #: 415-369-0679



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