Lymph Nodes that Clump

From: JOY MUNDY (jmundy@immuno.imvs.sa.gov.au)
Date: Thu Jul 10 1997 - 16:04:22 EST

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Hi

Can anybody help me with a cell suspension solution that will inhibit
cell clumping. We disaggregate lymph node tissue for routine flow but
have heaps of trouble with cell clumping. We have tried Mg and Ca free
RPMI, also DPBS with DNAse to no avail. We keep blocking up the probe
of the XL which is a bit of a bother.

Willing to try anything!!!!!!!
Joy Mundy
Dept. Human Immunolgy
Institute of Medical & Veterinary Science
Adelaide, South Australia 5000
Ph 61-8-82287476; Fax 61-8-82324092
jmundy@immuno.imvs.sa.gov.au

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