We have been running some pH studies using SNARF-1 on myocytes. The investigator wants to use Nigericin to calibrate the pH measurments but we have some concern about contaminating the sample lines and flow cell in the flow cytometer. A very recent paper by P.H. Richmond & R.D. Vaughan-Jones (Pflugers Arch - Eur J Physiol, No. 254, 1997- I have only provisional page numbers) indicates that residual nigericin can strongly affect subsequent nigericin-free samples in a superfusion flow chamber. They developed several extensive cleaning procedures to remove the contaminating nigericin from their apparatus. I have searched through the flow literature and have found references in which nigericin is mentioned as a means of calibrating measurements, but have found nothing addressing contamination of the instrument or cleaning nigericin from tubing/flow cells. Does anyone have experience with this compound and is contamination a problem in a flow cytometric application? -- Wayne Green wgreen@genetics.utah.edu
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