Does your DPBS contain magnesium? Some do, some don't. DNAse only works in the presence of Mg++. We use DNAse I, 300 mcg/ml, (60,000 Dornase U/mg) to break all sorts of cell clumps. I can't remember it failing (but this may be a reflection on the biology of my memory, as much as the biology of cell clumps). Calman ---------- From: JOY MUNDY Sent: Thursday, July 10, 1997 12:49 PM To: cyto-inbox Subject: Lymph Nodes that Clump Hi Can anybody help me with a cell suspension solution that will inhibit cell clumping. We disaggregate lymph node tissue for routine flow but have heaps of trouble with cell clumping. We have tried Mg and Ca free RPMI, also DPBS with DNAse to no avail. We keep blocking up the probe of the XL which is a bit of a bother. Willing to try anything!!!!!!! Joy Mundy Dept. Human Immunolgy Institute of Medical & Veterinary Science Adelaide, South Australia 5000 Ph 61-8-82287476; Fax 61-8-82324092 jmundy@immuno.imvs.sa.gov.au
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