Cy3 can be excited at 488 nm, but not very efficiently; its excitation maximum is at about 540 nm, and its emission maximum is at about 565 nm. You could use a flow cytometer with any of the following light sources; the closer to 540 nm, the better: Argon ion laser; 515 or 528 nm (528 needs special optics) Krypton or mixed gas ion laser; 520 or 530 nm Any big system may have these (e.g., FACS Vantage, MoFlo, EPICS Frequency-doubled YAG laser; 532 nm Green He-Ne laser; 543 nm The green He-Ne's have often been installed on Coulter Elites; the B-D FACScount also has a green He-Ne but its software (CD4 counts only) is probably too inflexible to make this an option. Mercury arc lamp; 546 nm These can be found on B-D's old FACS Analyzer, Partec's instruments, and the Bio-Rad Bryte (or the old Skatron). All the sources mentioned above have been used at one time or another on Cytomutts as well...but it doesn't pay to build or buy an instrument or maybe even a laser for one antibody. Why does this person need to use Cy3? -Howard
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:54 EST