Greatings all, I have been looking into the economics of our flow setup and discovered that I can buy PECy5 anti CD45 for much cheaper than I currently buy PerCP anti CD45. I proceeded to make some three color CD3/4/45 and 3/4/45 PBL's to do some compensation with and found that I got into real trouble :( Did something drastic go wrong with the labeling of the PBL's ??, or is there something I need to know about PE-Cy5 on a Calibur (ie: has anyone else done this easily/with difficulty ??) One problem is that I have very little quality information on the Calibur - the manual seems to make no mention of the of exactly which mirrors/filters the Calibur uses ... does it use a 650LP filter on the FL3 detector the same as a FacsScan does ?? (if that's the case then I'm only just picking up the *tail* of the PE-Cy5 emission) Peter Chapple Melbourne AUSTRALIA
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