There is an immunomagnetic method. See Jones, P.P., Peter, A.T., and Robinson, J.P.: An immunomagnetic method to separate X and Y chromosome bearing mammalian spermatozoa. Proc.Int.Cong.Anim.Reprod.Artif.Insem. 1:484-486, 1992. > Using an orienting flow nozzle and staining for DNA content using > Hoechst 33342 is the only reliable way to sort viable sexed sperm. > While the idea of using oligos makes sense, the problem is that you > have to kill the sperm to get the oligos into the DNA. No one to my > knowledge has been able to come up with any specific surface markers > that could be used to distinguish X from Y sperm without having to > fix the sperm. > > Mike Fox > > On Mon, 16 Jun 97 11:56:27 -0600 CHELACK@admin3.usask.ca wrote: > > > > > What kind of methods are being used to sort viable X and sperm in particular > > bull sperm. A graduate student has brought me some older literature > > describing sorting on differences in DNA content using specialized adaptors > > to maintain consistent orientation of the sperm as they pass through the flow > > cell. Is it possible to use FITC oligos to specific regions of the Y > > chromosome to accomplish this? I have sent the graduate student back to search > > out more on this possibility, but am wondering if anyone out there is already > > doing this or has found it to be impossible, or is using another even more > > elegant method? The graduate student is quite keen and quite green so I am > > trying to make sure he isn't biting off more than he can chew. > > > > > > regards > > > > > > BJC - Brian > > Mike Fox > Chairman, Cell and Molecular Biology Graduate Program > Dept. of Radiological Health Sciences mfox@cvmbs.colostate.edu > Colorado State University Ph: (970)491-7618 > Ft. Collins, CO 80523 USA Fax:(970)491-0623 > ==================================== >
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