Does this mean that there is an Ab against murine CD83 available?? Thanx. Paul J Jansen On Mon, 9 Jun 1997, Kevin G Waddick wrote: > Date: Mon, 9 Jun 1997 17:24:57 -0500 (CDT) > From: Kevin G Waddick <waddi002@maroon.tc.umn.edu> > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Cc: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: Re: double label for dendritic cells and MVE virus antigen > > > > If the infected cells co-express HLA-DR and CD83, they are dendritic cells. > > Kevin G. Waddick, Ph.D. > Staff Scientist > Hughes Institute > 2685 Patton Road > Roseville, Minnesota 55113 > (612) 628-0598 > (612) 628-9891 Fax > > On Mon, 9 Jun 1997, Leanne Sammels wrote: > > > > > Hello, > > > > I'm not sure that this is the right place to post this question > > but here goes. We have used in situ hybs to localise Murray > > Valley encephalitis virus RNA in the lymph nodes of mice > > infected IP with MVE. Only a small population of cells > > within the lymph node are positive for viral RNA and the > > morphology suggests it may be the follicular dendritic > > cells. I am interested in using flow cytometry to analyse > > infected lymph node cell suspensions(or maybe just > > peritoneal washouts?)to see if it is the dendritic cells > > which are replicating virus or if other cell populations > > are also involved in viral replication. Is this feasible? > > and if so can anyone recommend some publications which > > would be helpful. I'm sure it is quite obvious by now > > that I have had no cytometry experience whatsoever. Any > > advice would be appreciated. > > Kind regards, > > > > Lee Sammels > > Dr Leanne Sammels > > Department of Microbiology > > University of Western Australia > > Nedlands WA 6009 > > > > Phone: 08 9346 4872 > > Fax: 08 9346 2912 > > >
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:49:50 EST