I guess that I wasn't thinking as clearly as I should have been. The anti-CD83 MoAb we use is against the human antigen; I don't know if Coulter/Immunotech (from which we got it) has a murine version, or if the antibody cross-reacts. Kevin G. Waddick, Ph.D. Staff Scientist Hughes Institute 2685 Patton Road Roseville, Minnesota 55113 (612) 628-0598 (612) 628-9891 Fax On Tue, 10 Jun 1997, Paul J. Jansen wrote: > Does this mean that there is an Ab against murine CD83 available?? > Thanx. > Paul J Jansen > > > On Mon, 9 Jun 1997, Kevin G Waddick wrote: > > > Date: Mon, 9 Jun 1997 17:24:57 -0500 (CDT) > > From: Kevin G Waddick <waddi002@maroon.tc.umn.edu> > > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > > Cc: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > > Subject: Re: double label for dendritic cells and MVE virus antigen > > > > > > > > If the infected cells co-express HLA-DR and CD83, they are dendritic cells. > > > > Kevin G. Waddick, Ph.D. > > Staff Scientist > > Hughes Institute > > 2685 Patton Road > > Roseville, Minnesota 55113 > > (612) 628-0598 > > (612) 628-9891 Fax > > > > On Mon, 9 Jun 1997, Leanne Sammels wrote: > > > > > > > > Hello, > > > > > > I'm not sure that this is the right place to post this question > > > but here goes. We have used in situ hybs to localise Murray > > > Valley encephalitis virus RNA in the lymph nodes of mice > > > infected IP with MVE. Only a small population of cells > > > within the lymph node are positive for viral RNA and the > > > morphology suggests it may be the follicular dendritic > > > cells. I am interested in using flow cytometry to analyse > > > infected lymph node cell suspensions(or maybe just > > > peritoneal washouts?)to see if it is the dendritic cells > > > which are replicating virus or if other cell populations > > > are also involved in viral replication. Is this feasible? > > > and if so can anyone recommend some publications which > > > would be helpful. I'm sure it is quite obvious by now > > > that I have had no cytometry experience whatsoever. Any > > > advice would be appreciated. > > > Kind regards, > > > > > > Lee Sammels > > > Dr Leanne Sammels > > > Department of Microbiology > > > University of Western Australia > > > Nedlands WA 6009 > > > > > > Phone: 08 9346 4872 > > > Fax: 08 9346 2912 > > > > > >
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