If the infected cells co-express HLA-DR and CD83, they are dendritic cells. Kevin G. Waddick, Ph.D. Staff Scientist Hughes Institute 2685 Patton Road Roseville, Minnesota 55113 (612) 628-0598 (612) 628-9891 Fax On Mon, 9 Jun 1997, Leanne Sammels wrote: > > Hello, > > I'm not sure that this is the right place to post this question > but here goes. We have used in situ hybs to localise Murray > Valley encephalitis virus RNA in the lymph nodes of mice > infected IP with MVE. Only a small population of cells > within the lymph node are positive for viral RNA and the > morphology suggests it may be the follicular dendritic > cells. I am interested in using flow cytometry to analyse > infected lymph node cell suspensions(or maybe just > peritoneal washouts?)to see if it is the dendritic cells > which are replicating virus or if other cell populations > are also involved in viral replication. Is this feasible? > and if so can anyone recommend some publications which > would be helpful. I'm sure it is quite obvious by now > that I have had no cytometry experience whatsoever. Any > advice would be appreciated. > Kind regards, > > Lee Sammels > Dr Leanne Sammels > Department of Microbiology > University of Western Australia > Nedlands WA 6009 > > Phone: 08 9346 4872 > Fax: 08 9346 2912 >
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