Hello, I'm not sure that this is the right place to post this question but here goes. We have used in situ hybs to localise Murray Valley encephalitis virus RNA in the lymph nodes of mice infected IP with MVE. Only a small population of cells within the lymph node are positive for viral RNA and the morphology suggests it may be the follicular dendritic cells. I am interested in using flow cytometry to analyse infected lymph node cell suspensions(or maybe just peritoneal washouts?)to see if it is the dendritic cells which are replicating virus or if other cell populations are also involved in viral replication. Is this feasible? and if so can anyone recommend some publications which would be helpful. I'm sure it is quite obvious by now that I have had no cytometry experience whatsoever. Any advice would be appreciated. Kind regards, Lee Sammels Dr Leanne Sammels Department of Microbiology University of Western Australia Nedlands WA 6009 Phone: 08 9346 4872 Fax: 08 9346 2912
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