Dear FLOW experts: This is to ask your opinions about using FL-height, instead of FL-area in cell cycle analysis. I can see typical picture of G1, S and G2/M from FL- height histogram as long as I acquire data in linear mode. I am wondering if this is an acceptable way to do cell cycle analysis without purchasing the pulse processing module. The cytometer I am using is the BD Vantage. I appreciate your responses. Fang-Yao Hou FYHOU@macc.wisc.edu
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