pulse processing in cell cycle analysis

From: Fang-Yao Hou (FYHOU@macc.wisc.edu)
Date: Tue May 06 1997 - 19:34:00 EST


Dear FLOW experts:
 
This is to ask your opinions about using FL-height, instead of FL-area in
cell cycle analysis. I can see typical picture of G1, S and G2/M from FL-
height histogram as long as I acquire data in linear mode. I am wondering
if this is an acceptable way to do cell cycle analysis without purchasing
the pulse processing module. The cytometer I am using is the BD Vantage.
I appreciate your responses.
 
Fang-Yao Hou
FYHOU@macc.wisc.edu



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