In response to Fang-yao Hou, peak height measurements can under measure total fluorescence when the particle being measured has a cross-sectional diameter larger than the effective illumination spot of the laser. in cell-cycle analysis this situation will result in a mean channel G2/G1 ratio less than the theoretical 2.0 and often as low as 1.8. Therefore, pulse area measurements are preferred when doing DNA staining. On Tue, 6 May 1997, Fang-Yao Hou wrote: > > Dear FLOW experts: > > This is to ask your opinions about using FL-height, instead of FL-area in > cell cycle analysis. I can see typical picture of G1, S and G2/M from FL- > height histogram as long as I acquire data in linear mode. I am wondering > if this is an acceptable way to do cell cycle analysis without purchasing > the pulse processing module. The cytometer I am using is the BD Vantage. > I appreciate your responses. > > Fang-Yao Hou > FYHOU@macc.wisc.edu >
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