Re: Sorting into Terasaki plates

From: Jim Houston (jim.houston@stjude.org)
Date: Mon Mar 03 1997 - 11:57:08 EST


On Sat, 1 Mar 1997 ClaudeC81@AOL.COM wrote:

> 
> I have been asked to test / set up our single cell deposition system for
> sorting single cells into Terasaki plates.  Sounds like a challenge to me,
> but can it be done?  FACStar Plus, ACDU, macrosort head.  Isn't that sorted
> drop going going to make like a giant meteor on earth and blow all to well?
>  Is the sorter accurate enough to spit a drop / cell into such a small well /
> volume in the first place?  Answers to these and other questions would be
> much appreciated, as well as tips on how to do it... if it can be done!
>  Thanks, Claude
> 

I have done it on a Vantage with a TSO.  It takes a bit of time to adjust
the well differences.  Be patient.  It is not if the sorter can do it, but
can the operator?  Use 10-12 psi sample pressure.

Good luck,

Jim



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