Frank, try using PBS or any other uncolored medium as sheeth fluid, we found that the BDIS sheath (as also Coulters and some others) modify/inhibit the growth of some sensitive cellines after sorting (I suppose its some growthinhibitors or stabilizers in the commercial fluids). Might not be the reason, but could be worth a trial. Cheers, Matthias _____________________________________________________________________________ Matthias Haury Flowcytometry Dept Immunology Institut Pasteur mhaury@pasteur.fr Tel: 33 (01) 40 61 31 29 Fax: 33 (01) 45 68 86 39 _____________________________________________________________________________ >dear colleagues > >does anyone have experience sorting fibroblast cell lines? > >i'm sorting FLST cell line. this is an embryonic cell line which supports >b-cell development. >unfortunately after sorting the cells remain alive but don't attach like they >normally do > >i detach the cells by cell scraping and filter through a mesh post two >step staining. > >cytometry is on a facsvantage, 70 micron nozzle, sheath p.s.i is 11. > >any ideas for increasing viablity/adherence would be greatly appreciated. > > >frank isdell >flow cytometry lab. >the rockefeller university >1230 york ave >ny
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