dear colleagues does anyone have experience sorting fibroblast cell lines? i'm sorting FLST cell line. this is an embryonic cell line which supports b-cell development. unfortunately after sorting the cells remain alive but don't attach like they normally do i detach the cells by cell scraping and filter through a mesh post two step staining. cytometry is on a facsvantage, 70 micron nozzle, sheath p.s.i is 11. any ideas for increasing viablity/adherence would be greatly appreciated. frank isdell flow cytometry lab. the rockefeller university 1230 york ave ny
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