You can use a dye called Sytox Green (Cat # S-7020) sold by Molecular Probes which will enters dead cells and makes them intensely green fluorescent. It is excited by the same 488 line of the argon ion laser and hence can be used along with PE-conjugated Ab. >Adrian Moris wrote: > > Dear Flow Folks: > > A Co-worker and I are are wondering if there is a way to stain cells > for flow which will distinguish between live and dead cells when > co-stained with a PE conjugated Ab. Does anyone have such a protocol > they would share? ---------------------------------------------- Mahesh K. Bhalgat, Ph.D. Molecular Probes, Inc. Eugene OREGON 97402 Phone: (541) 465-8300 Fax: (541) 344 6504 E-mail: mahesh@probes.com -----------------------------------------------
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